Team:NYU Abu Dhabi/Documentation/DOCS 20ee279bfcdc46b09c4fb108851b2757/Engineering 8297e0cf88af4f8fbfe00ad598c6a52f/Bio-Requirements a0409b5140a944dc8b04a55669a38016

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Bio-Requirements

• Working Protocol
  • Sample
    • eDNA
    • Swab
  • DNA Extraction and Purification
    • Lysis Buffer
    • Bead Beating
  • Amplification
    • RPA/LAMP
    • Heating (Thermocycling?)
  • Reporting
    • If Cas 12 → Quench Single stranded DNA Reporters
    • If Cas 13 →Quench Single stranded RNA Reporters
    • Fluorescence
    • Lateral Flow Readout using FAM-Biotin Reporter
    • Electrochemical sensing

What we don't have right now:

• Volumes of anything

• Reaction temperatures

• More definition on protocol options

Questions

Name	Created	Tags
Sample Extraction & Input	@Oct 27, 2020 6:42 PM
Amplification & Detection	@Oct 27, 2020 6:42 PM
Communication of the Results	@Oct 27, 2020 6:42 PM